Experiments on the formation of carboxylase and thiamine pyrophosphate in living bakers' yeast
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چکیده
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THE CALCIUM BINDING SITES OF THE BAKERS' YEAST TRANSKETOLASE
The calcium binding sites of Bakers' Yeast Transketolase (TK) was elucidated by estimating the pKa values of the functional groups that bind to calcium. These pKa's were found to be 6.25 and 7.2 relating to the pKa's of the two immidazol moieties of histidine residues on the enzyme. The rate of the binding of calcium to the enzyme was obtained separately as a function of pH. Maximum values ...
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15 صفحه اولInhibition of Thiamine Pyrophosphate Utilization by Thiamine
The growth of a thiamine pyrophosphate auxotroph of Escherichi coli was inhibited by either thiamine or thiamine monophosphate, and the growth of a thiamine monophosphate auxotroph was inhibited by thiamine. The thiamine pyrophosphate-dependent oxidation of pyruvate was inhibited by thiamine with whole cells of the thiamine pyrophosphate auxotroph but not with cell extracts prepared from the sa...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta
سال: 1951
ISSN: 0006-3002
DOI: 10.1016/0006-3002(51)90070-4